Template Concensus Sequence Pipeline
DESCRIPTION General application to create template concensus sequences (TCS) for single or multiplexed pair-end Primer ID (PID) MiSeq sequencing.
Primer ID Lib Prep Protocol
Please cite TCS pipeline Version 2.5.#
Zhou S, Jones C, Mieczkowski P, Swanstrom R. 2015. Primer ID Validates Template Sampling Depth and Greatly Reduces the Error Rate of Next-Generation Sequencing of HIV-1 Genomic RNA Populations. J Virol 89:8540-55. https://www.ncbi.nlm.nih.gov/pubmed/26041299
MiSeq Format